Essential function of transmembrane transcription factor MYRF in promoting transcription of miRNA lin-4 during C. elegans development

Precise developmental timing control is essential for organism formation and function, but its mechanisms are unclear. In C. elegans, the microRNA lin-4 critically regulates developmental timing by post-transcriptionally downregulating the larval-stage-fate controller LIN-14. However, the mechanisms triggering the activation of lin-4 expression toward the end of the first larval stage remain unknown. We demonstrate that the transmembrane transcription factor MYRF-1 is necessary for lin-4 activation. MYRF-1 is initially localized on the cell membrane, and its increased cleavage and nuclear accumulation coincide with lin-4 expression timing. MYRF-1 regulates lin-4 expression cell-autonomously and hyperactive MYRF-1 can prematurely drive lin-4 expression in embryos and young first-stage larvae. The tandem lin-4 promoter DNA recruits MYRF-1GFP to form visible loci in the nucleus, suggesting that MYRF-1 directly binds to the lin-4 promoter. Our findings identify a crucial link in understandi..., , , # Data from: Essential function of transmembrane transcription factor MYRF in promoting transcription of miRNA lin-4 during C. elegans development

[https://doi.org/10.5061/dryad.tqjq2bw71](https://doi.org/10.5061/dryad.tqjq2bw71)

The dataset comprises original images in CZI, TIF, or VSI formats, and datasheets in XLSX or XLS formats from quantitative and statistical analysis. The folder and file names typically indicate essential genotype and animal stage information. The files may be supplemented by additional notes in TXT format.

CZI files were acquired using a Zeiss LSM880 microscope with Airyscan.
VSI or TIF files were acquired using an Olympus BX63 microscope.
Excel files (XLSX/XLS) are used for organizing and presenting data summaries and results of quantitative analyses.

## Description of the data and file structure

Figure 1A: The dataset consists of a collection of representative images illustrating the subcellular localization patterns of GFP::MYRF-1 and the expression of ...