High-throughput sequencing analysis of mRNA and lncRNA in intestinal epithelial cells of wild-type mice, germ-free mice and wild-type mice infected with S. Typhimurium

At the epigenetic level, lncRNAs play important roles in cell differentiation and function. We identified novel long-stranded non-coding RNA (GM1082) in intestinal epithelial cells.We used high-throughput sequencing to detail the global programme of gene expression and identified distinct classes of up or down regulated genes in intestinal epithelial cells of wild-type mice, germ-free mice and wild-type mice infected with S. Typhimurium. Overall design: Intestinal epithelial cells were isolated by incubating in HBSS containing 2 mM EDTA for 30 min and shaking.