hMDH1 Oxaloacetate Kinetic Data

Shown are fits to kinetic data of human cytoplasmic malate dehydrogenase (hMDH1), UniProt ID P40925, with constant concentration of NADH and varying concentrations of oxaloacetate (OAA). On the left, the data is fit to the classic Michaelis-Menten equation, and the determined values of kcat, Km, and kcat/Km are provided. The curve does not follow the data, so a modified equation using Hill coefficients is used to fit to the data, as shown to the right in the figure. This equation provides an exponent "n" to the substrate concentration and to Km in the Michaelis-Menten equation. The Km term is renamed to Khalf, but these would be equivalent if n=1.