Ct-values of A1M, HO-1 and p21 mRNA expression.
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cDNA was prepared using reverse transcription and quantified by real-time PCR as described in Materials and Methods. A1M, HO-1 and p21 threshold cycle values were normalized against G3DPH. Results are from triplicate experiments and are presented as mean ± SEM. Statistical comparison between groups was made using Students t test. * PPP1 Statistical comparison vs. control, 2 statistical comparison vs heme or Fenton-reaction. The Ct-values were also used to calculate ΔΔCt-values and these are shown in Figures 1 and 2.
创建时间：
2015-12-02
