Transcriptome analisys of bone marrow mononuclear cells derived from pediatric patients with acute myeloid leucosis. Homo sapiens

The aim of this research was to explore activation/deactivation of signaling pathways in mononuclear cells from pediatric patients with acute myeloid leucosis and acute lymphoid leucosis. Overall design: Mononuclear cells were separated shortly after bone marrow samples collection. Cells were obtained by a density gradient centrifugation method using Ficoll. Cells were dissolved in RNALater solution, aliquoted and stored at -20°C till RNA extraction and microarray hybridisation.