Time-series RNAseq analysis following lateral root induction by gravistimulation

We report the comparison of transcriptomic profiles in specific lateral root tissues for Col-0 wild type and puchi-1 mutant seedlings. Lateral root organogenesis is a key process in plant root system development and adaptation to the environment. To dissect the molecular events occurring during the early phase, we generated time-series transcriptomic datasets profiling lateral root development in puchi-1 and wild type backgrounds. Consistent with a mutually inhibitory mechanism, transcriptomic and reporter analysis revealed meristem-related genes were ectopically expressed during early stages of lateral root primordium formation in puchi-1. We conclude that PUCHI participates to the coordination of lateral root patterning and represses ectopic establishment of meristematic cell identities during early stages of organ development. Overall design: The experiment comprises 36 samples: 2 genotypes (Col-0 wild type and puchi-1 mutant), and 6 treatments (no treatment, 12h, 18h, 24h, 30h and 36h gravistimulation), with 3 replicates per condition (genotype and treatment).