DNA methylation analyses of HygR colonies obtained in cotransformations of N644 with hH3 alleles.

1Fraction transformations that gave rise to HygR colonies.2Fraction transformations that showed loss of DNA methylation in Southern blotting.3One transformant out of 12 tested exhibited hypomethylation.4Of the two HygR transformants obtained, both were methylated.5Half of the transformants tested (28) demonstrated loss of DNA methylation.6All HygR transformants tested were methylated.7Two HygR transformants out of 11 tested were hypomethylated.