Expression of rhEPOFc in N. benthamiana.

Concentration of transiently expressed rhEPOFc was determined using a commercially available immunoassay. For each sample the concentration is given in mg/kg of fresh leaf. The percentage of the total soluble protein (TSP) was also calculated. rhEPOFc was expressed in N. benthamiana ΔXTFT mutants (ΔXTFT); co-expressed in ΔXTFT with mammalian genes for protein sialylation (Sia); co-expressed in ΔXTFT with mammalian genes for synthesis of tri-antennary sialylated N-glycans (TriaSia) and co-expressed in ΔXTFT with mammalian genes for synthesis of tetra-sialylated N-glycans (TetraSia)