Microbiome of two Amazon freshwater sponges, Drulia brownii and Tubella paulula

For the project, sampling was conducted in the Tapajs River (Santare, Para, Brazil), a clear-water, oligotrophic system strongly influenced by Amazonian seasonality. Two contrasting sites were selected: Praia do Cajueiro, an urbanized and anthropogenically impacted freshwater beach, and Ponta do Cururu, a non-urbanized site located approximately 4.7 km away. Collections of adult sponges were performed during two hydrological extremes of the Amazon flood pulse: the rainy season (July 2023) and the dry season (November 2023). DNA was extracted from bulk sponge tissue and gemmules using the FastDNA SPIN Kit for Soil, without prior gemmule sterilization to preserve epibacterial communities. DNA quality was assessed by 1% agarose gel electrophoresis. Two sponge species consistently represented across samples were selected for shotgun metagenomic sequencing, resulting in 18 libraries (two species, two seasons, and three biological replicates per condition). Sequencing was performed on an Oxford Nanopore PromethION P2 platform. The raw sequencing reads generated in this study have been deposited in the National Center for Biotechnology Information (NCBI) BioProject database under accession number PRJNA1420500. Furthermore, the 23 Metagenome-Assembled Genomes (MAGs) recovered from the sponge microbiomes, along with the Cytochrome c Oxidase subunit I (COI/CO1) gene sequences used for the taxonomic identification of all sponge individuals, are publicly available via this Zenodo deposit.