Mycobacterium tuberculosis isolate 13-4152 genome sequencing

Mycobacterium tuberculosis isolate 13-4152 was isolated in 2013 from 11 years old male patient from Moscow, Russia, with diagnosed caseous pneumonia. This strain was resistant to isoniazid, rifampicin, pyrazinamide, ethionamide, amikacin and capreomycin according to Bactec MGIT 960 DST. This strain lacks mutations in known PZA resistance genes (pncA, rpsA, panD) and has positive PZase activity, though its resistance to PZA was confirmed 3 times by DST. This genome may elucidate new genetic mechanisms of PZA resistance.