Data for: Probing the architecture, dynamics, and inhibition of the PI4KIIIA/TTC7/FAM126 complex

Table S1A. All hydrogen deuterium exchange (HDX) peptide data for experiments examining the global exchange of PI4KIIIA, TTC7B, and FAM126A. The charge state (Z), residue start, residue end number, retention time (RT) and sequence are displayed for every peptide. In the Raw Data column, the two time points (0.3s and full) are labelled, and the relative level of HDX is coloured according to the amount of deuterium incorporated, on a blue to red continuum. The data listed for the 0.3s time point are the average of three independent experiments, with SD shown next to all HDX values. In the Normalized to Full Deuteration column, the 0.3s data has been normalised to the full deuteration measurements with the exception of those data (surrounded by black lines) where the full deuteration measurement was lower than 20% deuterium incorporation. The third column denotes the corresponding peptide centroid.

Table S1B. All HDX peptide data for experiments examining the complex dynamics of PI4KIIIA, TTC7B, and FAM126A. The charge state (Z), residue start, residue end number, retention time (RT) and sequence are displayed for every peptide. The two columns represent each state examined (+/- PI4KIIIA) and contain the data for five time points. The data listed are the average of three independent experiments, with SD shown next to all HDX values.

Table S1C. All HDX peptide data for experiments examining the dynamics of inhibitor specificity of PI4KIIIA, TTC7B, and FAM126A. The charge state (Z), residue start, residue end number, retention time (RT) and sequence are displayed for every peptide. The three columns represent each state examined (+/- inhibitor) and contain the data for four time points. The data listed are the average of three independent experiments, with SD shown next to all HDX values.

表S1A：展示了对PI4KIIIA、TTC7B和FAM126A全局交换进行考察的所有氢氘交换（HDX）肽段数据。对于每个肽段，均展示了电荷状态（Z）、氨基酸起始位置、氨基酸终止位置编号、保留时间（RT）以及序列。在原始数据栏中，标记了两个时间点（0.3秒和完整）的数据，并根据氘的掺入量以蓝红渐变的方式着色表示HDX的相对水平。0.3秒时间点的数据为三次独立实验的平均值，所有HDX值旁边均显示标准差。在归一化至完全氘代栏中，0.3秒的数据已归一化至完全氘代测量值，但黑线包围的数据除外，这些数据的完全氘代测量值低于20%的氘掺入。第三列表示相应的肽段质心。

表S1B：展示了对PI4KIIIA、TTC7B和FAM126A复杂动态进行考察的所有HDX肽段数据。对于每个肽段，均展示了电荷状态（Z）、氨基酸起始位置、氨基酸终止位置编号、保留时间（RT）以及序列。两列代表考察的每个状态（+/−PI4KIIIA），包含五个时间点的数据。所列数据为三次独立实验的平均值，所有HDX值旁边均显示标准差。

表S1C：展示了对PI4KIIIA、TTC7B和FAM126A抑制剂特异性动态进行考察的所有HDX肽段数据。对于每个肽段，均展示了电荷状态（Z）、氨基酸起始位置、氨基酸终止位置编号、保留时间（RT）以及序列。三列代表考察的每个状态（+/−抑制剂），包含四个时间点的数据。所列数据为三次独立实验的平均值，所有HDX值旁边均显示标准差。