Construction of a novel UDP-rhamnose regeneration system by a two-enzyme reaction system and application in glycosylation of flavonoid

In summary, we provided a novel fusion enzyme (VvRHM-NRS), which was NADPH-independent and could convert UDP-glucose to UDP-rhamnose with NADH self-sufficient. A cofactor self-sufficient UDP-rhamnose regeneration system was developed by VvRHM-NRS coupled with GmSUS. By optimizing coupled reaction conditions, a total of 0.57 mM UDP-rhamnose was generated from sucrose, NAD+, and UDP. This synergistic system provides a green chemistry approach for the UDP-rhamnose regeneration in glycosylation of flavonoids and other bioactive substances.

搜集汇总

背景与挑战

背景概述

该数据集描述了一种新型融合酶VvRHM-NRS，它能够不依赖NADPH而利用NADH自给将UDP-葡萄糖转化为UDP-鼠李糖。通过将该酶与蔗糖合成酶GmSUS耦合，构建了一个辅因子自给的UDP-鼠李糖再生系统，优化后从底物生成了0.57 mM UDP-鼠李糖，为黄酮类化合物糖基化提供了绿色化学方法。