Effect of mannose supplementation on high fat diet fed mice.

Using a C57BL6/J mouse model of diet-induced obesity, we observed that mannose (M) supplementation of high fat diet (HFD)-fed mice prevents weight gain, lowers adiposity, reduces liver steatosis, and improves glucose tolerance and insulin sensitivity. These beneficial effects of mannose are observed when supplementation is started early in life (0 and 3 weeks post weaning) but are lost when started later in life (8 weeks post weaning). We did 16S metagenomic analysis of gut microbiota from Normal diet (ND), HFD and HFD+M mice to understand the changes in microbial composition.