RNAseq data for Yarrowia lipolytica strains (Po1h derivatives with heterologous secretory protein overproduction)

The primary purpose of this study was identification of bottlenecks within the translational-secretory machinery of a nonconvential yeast species Yarrowia lipolytica. Yl is a promising expression host, frequently used as a heterologous protein production platform. As evidenced previously, Yl's secretory pathway is characterized by several specificieties that make it particularly efficient. Nevertheless, upon high overexpression of a heterologous gene encoding a secretory protein, secretion is the limiting stage of the synthesis process.Methodological concept was to generate Yl strains with differentially burdened secretory pathway by cloning 4 different heterologous genes, encoding biochemically different proteins (plus a prototrofic reference strain). Four different heterologous proteins were chosen as the models: 50 kDa enzymatic secretory protein with 35696 disulfide bond combinations (SoA), 65 kDa enzymatic secretory protein with 21 predicted N-/O-glycosylation sites (TlG), 27 kDa small fluorescent secretory protein with a negligible number of post-translational modifications (scYFP), small intracellular protein (inYFP) as a control.All the strains were cultivated in chemostat bioreactor cultures for reliable assessment of DEGs (differentially expressed genes). RNAseq data were generated by NGS Illumina sequencing of total RNA extracted from the 5 strains maintained in the chemostat cultures.