Relative luciferase activity 24 h after transfection of the various cell lines with constructs with lesions at codons 5, 297 and 445.

aNTS: nontranscribed strand; TS: transcribed strand ; G*: 8OG.
bValues are expressed as [(RLUPp/RLURr)construct/(RLUPp/RLURr)100%]×100 in the same cell line. Each value is the mean of at least six replicate samples. RLU: relative light units.
cp values for the Mann-Whitney U test are 0.0374 and 0.0250 for comparisons with the relative luciferase activities of MRC5V1 or VA13 cells transfected with the same construct, respectively.
dDistributions were considered to be significantly different when p<0.05.
eBoth p values for the Mann-Whitney U test are <0.0001 for comparisons with the relative luciferase activities of MRC5V1 or VA13 cells transfected with the same construct.
fp values for Mann-Whitney U test are <0.0001 and 0.0022 for comparisons with the relative luciferase activities of MEF cells transfected with (8OG/Stop)5 and (8OG/Stop)297, respectively.
gThe dose of doxycycline was reduced from 2 µg/ml to 1 ng/ml.