Reversibility of the inhibition.

Developing D. discoideum cells were incubated on filters on top of membranes containing D. caveatum or buffer (control) for 24 hours. Filters were then transferred to buffer-filled membranes and scored after 24, 48 and 72 hours. Each score represents the number of aggregates as a percentage of the total number of developmental stages observed at the time indicated (average (standard deviation)), as illustrated in Figure 5. The development inhibition is reversible: ∼75% of the D. caveatum-inhibited D. discoideum aggregates complete development to fruiting when rescued on SorC buffer for 72 hours.