Molecular profiling of mouse septal neurons with single nucleus RNA-seq. Molecular profiling of mouse septal neurons with single nucleus RNA-seq

To reveal functional diversity of neurons in the septal area of the forebrain, we isolated neuron nuclei from septal tissues of wild-type mice by FACS sorting and subjected them to single nucleus (sn)RNA sequencing with the 10x platform. Overall design: NeuN+ nuclei from the septal area of adult C57BL6/J mice were purified and subjected to snRNA-seq