The expression changes of small RNA during BMSCs proliferation

The proliferation, migration, and osteogenic differentiation of bone mesenchymal stem cells (BMSCs) play vital roles in maintaining bone mass and metabolism. BMSC base cell therapy has become a competitive treatment option for osteoporosis.Small are critical regulators of cellular processes. Small RNA sequencing was used to detect piRNA expression during BMSC proliferation. Overall design: The serum-free test (ST) group cells were cultured in a serum-free medium for 24 h, and the general medium (GM) group cells were cultured in a medium containing 10% fetal bovine serum for 24 h. sequencing.