CRISPR/Cas9 genome editing demonstrates that CCM3 modulates endothelial miRNA expression
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE114947
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资源简介：
CCM3 regulates blood-brain-barrier integrity and vascular maturation in vivo. CCM3 loss-of-function variants predispose to cerebral cavernous malformations (CCM). Various signalling pathways are deregulated upon CCM3 depletion in endothelial cells (ECs). In this study, we established a crRNA:tracrRNA:Cas9 RNP approach to efficiently knockout CCM3 in human ECs and studied the molecular and functional effects of its long-term inactivation. Using small RNA sequencing, we show that CCM3 regulates the expression of aging‑associated miRNAs. Examination of the miRNA expression profile of 4 wild-type CI-huVEC lines and 4 clonal CCM3-knockout CI-huVEC lines
创建时间：
2019-01-25
