The cooperation among the core regulatory circuitry transcription factors and MYCN on DNA binding [HAND2-MYCN-CRC-knockdown_ChIPseq]

MYCN and the core regulatory circuitry transcription factors HAND2, PHOX2B and GATA3 cooperate to bind to DNA. IMR32 cells were transfected with control siRNA (siCtrl), or HAND2 siRNA (siHAND2), or MYCN siRNA (siMYCN), or GATA3 siRNA (siGATA3), or PHOX2B siRNA (siPHOX2B), and cultured in regular medium for 24 hr or 72 hr, then ChIP experiments were performed using ChIP-IT High Sensitivity Kit (Active Motif ).