Mixed evidence for intralocus sexual conflict from male-limited selection in <em>Drosophila melanogaster</em>

Sexual conflict over shared traits – intralocus sexual conflict (IaSC) – may be common and consequential, but experimental tests of its relative magnitude are challenging and limited in number. We use a sex-limited selection experiment, designed to subject haplotypes of Drosophila melanogaster to selection for male fitness without opposing selection acting on female fitness. Importantly, we use three novel base populations to compare results with those from the LHM population, the sole population investigated using this technique. In contrast with previous studies, we find that the male fitness of haplotypes subject to male-limited selection (ML populations) is not consistently better than their matched (MC) controls when tested in the “wildtype” state. Males from ML lines did not outperform controls in competitive fitness assays, mate choice trials, fecundity induction, or sperm offense tests. As predicted, genetic variation for male fitness was reduced, with low fitness haplotypes app..., Full methodological details are provided in the associated publication. Breeding designs used to produce animals from various treatments can be found in the online supplement. Competitive reproductive fitness (CRF) was assayed for both males and females against competitive recessive (Cr) marker stocks in equal numbers. Test females were collected as virgins and test males at 10–11 days post-oviposition. On day 12, groups of 30 MC or ML flies were combined with 30 Cr of the same sex and 50 Cr of the opposite sex in ventilated “mini-cages” with yeast for 2 days. Six competition arenas (“contests”) were set up per population, and offspring proportions from six egg vials per arena were counted to estimate CRF. Fitness assays for hemiclonal analysis followed a similar design with reduced competition size to accommodate 76 lines. For males, 4 test flies were combined with 4 Cr males and 6 Cr females. For females, 4 virgins were combined with 3 Cr males, and productivity (pupal count) was us..., # Data from: Mixed evidence for intralocus sexual conflict from male-limited selection in *Drosophila melanogaster* ## Title of Dataset Wild-type estimates of fitness, sperm comp, mate choice, and heritable variation for fitness under male-limited experimental evolution. #### Associated Publication * **Title:** Mixed evidence for intralocus sexual conflict from male-limited selection in Drosophila melanogaster * **Journal:** Journal of Heredity * **Authors:** * Harshavardhan Thyagarajan, * Imran Sayyed, * Mindy G. Baroody, * Joshua A. Kowal, * Troy Day, * Adam K. Chippindale #### Dataset Overview This dataset contains experimental data on fitness, mate choice and sperm competition measurements for male-limited and control selection populations; and fitness measurements for hemiclones expressed male and female backgrounds. The data pertains to experimentally evolved *Drosophila melanogaster* populations. The data are provided as CSV files. The six files correspond to d...,