twk target sequences used in CRISPR/Cas9-based mutagenesis.

For all twk genes, two sgRNAs were used simultaneously. The use of twk-40 sgRNAs was as follows: twk-40a sgRNA # a1 and # a2 together with a repair template (S10 Table) for mac564; twk-40a sgRNA # a3 and # a4 for mac469, mac472 and neuron-specific knockouts; twk-40a sgRNA # a5 and # a6 together with a repair template for mac504 and mac505; twk-40b sgRNA # b1 and # b2 for mac554 and mac555; twk-40c sgRNA # c1 and # c2 for mac556 and mac557; twk-40b sgRNA # b2 and twk-40c sgRNA # c2 for mac558, mac559 and mac560. For each gene or twk-40 isoform, the number of transgenic lines examined, and the number of lines carrying the indicated mutations are shown. (XLSX)