Genome-wide analysis of replication fork locations in hydroxyurea-treated Saccharomyces cerevisiae by TrAEL-seq

TrAEL-seq was performed on hydroxyurea-blocked and then released yeast cells to track replication fork stalling and replication fork restart, in wild-type and replisome mutant strains. Wild type and replisome mutant cells were synchronized in G1 phase, released into 200mM HU to arrest all forks (T0 Timepoint) and then released the cells into normal media to analyze fork recovery 20 min (T1 timepoint) and 40 min (T2 timepoint) after the removal of hydroxyurea.