Supplemental datasets: Opsin gene expression plasticity and spectral sensitivity in male damselflies could mediate female colour morph detection

The visual systems of Odonata are characterized by many opsin genes, which form the primary light-sensitive photopigments of the eye. Female-limited colour polymorphisms are also common in Odonata, with one morph typically exhibiting male-like (androchrome) colouration and one or two morphs exhibiting female-specific colouration (gynochromes). These colour polymorphisms are thought to be maintained by frequency-dependent sexual conflict, in which males form search images for certain morphs, causing disproportionate mating harassment.  Here, we investigate opsin sensitivity and gene expression plasticity in mate-searching males of the damselfly Ischnura elegans during adult maturation and across populations with different female morph frequencies. We find evidence for opsin-specific plasticity in relative and proportional opsin mRNA expression, suggesting changes in opsin regulation and visual sensitivity during sexual maturation. In particular, expression of the long-wavelengt..., , , # Data and code for opsin function expression and qPCR analysis for I. elegans

[https://doi.org/10.5061/dryad.7sqv9s4z1](https://doi.org/10.5061/dryad.7sqv9s4z1)

We have submitted datasets with measured spectral sensitivity from UV-VIS spectrophotometry for the honey bee (Apis mellifera) short-wavelength sensitive (SW) opsin and for two wild-type damselfly (Ischnura elegans) SW opsins , a negative control I. elegans SW opsin, and 6 I. elegans long-wavelength sensitive (LW) opsins as well as R code to fit spectral sensitivity data to visual pigment templates (dataset 1); newick and fasta files used to generate a phylogeny for Ischnura spp. opsin sequences (dataset 2); dataset with relative expression values and proportion expression for tested opsins in I. elegans and R code used in primary data analysis (dataset 3).

## Description of the data and file structure

Dataset 1: Files associated with Fig 2 and Fig S2: A. mellifera SWS opsin functional expression, I. elegans SWS opsin fun...,