five

The beef cattle rumen microbiome composition is influenced by host genetics

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NIAID Data Ecosystem2026-04-25 收录
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https://figshare.com/articles/dataset/The_beef_cattle_rumen_microbiome_composition_is_influenced_by_host_genetics/25085600
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DNA was extracted from the rumen samples using the PowerMag Soil DNA isolation kit (MoBio Laboratories, Carlsbad, CA, USA) according to the manufacturer’s protocol with a few modifications as described by publication. The modified protocol also included adding RNase A to the lysis solution to ensure removal of RNA during DNA extraction. The isolated DNA was stored at -20°C until used for bacterial community analysis. The V4 region of the 16S rRNA gene was amplified and sequenced on the MiSeq platform.
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2019-07-04
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