Exogenous H2S administration does not regulate gene expression in primary murine alveolar type II cells

Primary alveolar type II cells were either treated with vehicle or NaHS for 6 h and 24 h (RS-266 samples). Primary alveolar type II cells treated with GYY4137 at 100 µM concentration were exposed to either 21% O2 or 85% O2 for 24 h (RS-284 samples). Overall no changes in gene expressionwere found in the corrected p values of both microarray studies. Four vehicle samples at 6 h of exposure, four NaHS treated samples at 6 h of exposure, four vehicle samples at 24 h of exposure and four NaHS treated samples at 24 h of exposure were used for the RS-266 microarray study. four GYY4137 100 µM samples in 21% O2 after 24 h of exposure were compared to four GYY4137 100 µM samples in 85% O2 after 24 h of exposure and used for the RS-284 microarray study.