Expression data from B6 mouse miR-142 KO and WT dendritic cells (DCs). Mus musculus

DCs are critical for regulating immune responses. miRNAs are small, noncoding RNAs and play a significant role in regulating immune responses. miR-142 is a hematopoietic specific miRNA. To explore the role of miR-142 in regulating DCs’ immune responses, we generated mutant mice bearing a targeted deletion of the miR-142 gene. We used microarrays to detail the global program of gene expression underlying the profile changes between miR-142 KO and WT DCs and identified distinct classes of up-regulated or down-regulated genes in steady state or LPS stimulation during this process. Overall design: miR-142 KO mice and WT littermates (biological triplicates) matched with age and sex were selected. DCs were purified from spleens by positive selection and processed for RNA isolation and hybridization on Affymetrix microarrays.