S1 File - Purification and Characterization of Recombinant N-Terminally Pyroglutamate-Modified Amyloid-β Variants and Structural Analysis by Solution NMR Spectroscopy

Method A. Cloning of recombinant plasmid encoding Aβ(E3Q-40/42) fusion protein. Method B. Expression and purification of Aβ fusion proteins. Method C. Cleavage of the fusion protein and purification of Aβ. Method D. Conversion to pEAβ40 and pEAβ42. Method E. pEAβ sample preparation. Method F. Mass spectrometry. Method G. Thioflavin-T assay. Method H. NMR spectroscopy. (DOCX)