Transcriptomic profiling change of MDA-MB-231 cells overexpressing DNAJC12

Purpose: DnaJ heat shock protein family (Hsp40) member C12 (DNAJC12) belongs to Hsp40/DnaJ protein family containing a J domain.The goal of this study is to compare the transcriptomic profiling change of MDA-MB-231 cells overexpressing DNAJC12 compared with MDA-MB-231 cells transfected with empty vector. Methods: 5×10^6 cells of each sample were collected, and total RNA was extracted using TRIZOL. After extraction, the RNA integrality was validated by Agilent 2100 bioanalyzer, and the qualified RNA was used to establish an RNA library, which was checked by quality control with Agilent 2100 bioanalyzer before sequencing. Transcriptome sequencing was achieved by Illumina Novaseq platform. MDA-MB-231 were divided into two groups transfected with DNAJC12v or empty vector separately, and each group contains 3 replicates.