Mouse glutamatergic neurons immunofluorescence images upon Suz12 isoforms deletions

The Polycomb repressive complex 2 (PRC2) mediates epigenetic maintenance of gene silencing in eukaryotes via methylation of histone H3 at lysine 27 (H3K27).
Alternative splicing (AS) of the PRC2 core component SUZ12 generates an uncharacterised isoform SUZ12-S, which co-exists with the canonical SUZ12-L isoform in the same cell in virtually all tissues and developmental stages. While SUZ12-S is necessary and sufficient to ensure correct repression of Polycomb target genes via promoter-proximal H3K27me3 deposition, SUZ12-L is needed for maintaining global H3K27 methylation levels. We showed that mouse embryonic stem cells (ESCs) lacking either of the two isoforms have a slower exit from pluripotency and fail to acquire neuronal cell identity upon differentiation stimuli. This dataset contains raw immunofluorescence images of ESCs derived neurons stained for H3K27me3 (RRID:AB_2614987), MAP2 (RRID:AB_2138153), and TUBB3 (RRID:AB_2564645). All images were counter-stained with DAPI before mounting.