Measurements and tif files for protocol: A surgical and experimental solution for minimizing off-target neuronal activation during optical stimulation within the mouse brain in vivo

Understanding the function of neuronal circuits in vivo often relies on optogenetic manipulation of neuronal populations using intracranial light stimulation at different wavelengths. However, recent experiments show that intracranial optical stimulation in mice can result in non-specific off-target activation of endogenous opsins located in the retina that potentially disrupts behavioral task performance Such off-target effects can arise from two primary sources: 1) direct light leakage from the implant and exposed skull (Figure 1A) and 2) light propagation through brain tissue to the retina (Figure 1B). Here we show data that displays the light leakage through the implants and eyes of mice under different conditions