Supplementary File 6. High-throughput miRNA-sequencing of the human placenta: expression throughout gestation

Aim: To understand
miRNA changes across gestation in healthy human placentae. This is essential
before miRNAs can be used as biomarkers or prognostic indicators during
pregnancy. Materials & Methods: Using next-generation sequencing, we
characterize the normative human placenta miRNome in first (N=113) and third
trimester (N=47). Results and Conclusions: There are 801 miRNAs expressed in
both first and third trimester, including 182 with similar expression across
gestation (P≥0.05, FC≤2) and 180 significantly different (FDR<0.05,
FC>2). Of placenta-specific miRNA clusters, C14MC decreases across gestation
and C19MC is overall highly expressed. Chromosome 13 clusters are upregulated in
first trimester. This work provides a rich atlas of healthy pregnancies to
direct functional studies investigating the epigenetic differences in first and
third trimester placentae.