Long read sequencing of nuclear RNAs from human coronary artery smooth muscle cells

To study isoforms of nuclear RNAs, including CARMN lncRNA, we performed Oxford Nanopore long-read sequencing of RNAs isolated from the nuclear fraction of human coronary artery smooth muscle cells. We perfomed Oxford Nanopore long read sequencing (MinION) on 2 biological replicates of RNAs isolated from nuclear fraction of HCASMCs.