Codon-based test of purifying selection averaging over all HBV sequence pairs in untreated or treated pregnant/ post-partum CHB carriers.

Notes:a7 cases underwent HBV sequence analysis in both pregnancy and post-partum in pre-S/S and/or pre-C/C region (160, 176, 196, 215, 223, 226, 233).bHBV sequence analysis in 4 cases (ID# 133, 150, 196–2 and 239) showed evidence of purifying selection. These cases also had ALT difference in pregnant compared to the post-partum period. (P-values less than 0.05 are significant).c Case #176 was followed during 2 pregnancies, HBV pre-S/S sequences were analyzed in a sample collected after her first pregnancy (176–2 post-partum) and during her second pregnancy (176–3). HBV. HBV Pre-C/C sequences were analyzed at 3 time-points during first pregnancy (176), post-partum (176–2) and during second pregnancy (176–3) (see Figs 1 and 2).ddS and dN are the numbers of synonymous and nonsynonymous substitutions per site, respectively. The probability of rejecting the null hypothesis of strict-neutrality (dN = dS) in favor of the alternative hypothesis (dN Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725–9).Codon-based test of purifying selection averaging over all HBV sequence pairs in untreated or treated pregnant/ post-partum CHB carriers.