Methods correlation between RT-PCR test and FA test sequencing at Site 2 and after 454 sequencing of discrepant specimens.

CI = confidence interval; MD = mutation detected; MND = mutation not detected.*Seven samples were reported as wild type by FA test and ‘mutation detected’ by RT-PCR test of which four were subsequently found to be V600E by 454, and three to be V600K. One sample was reported as V600G by FA test and ‘mutation detected’ by the RT-PCR test and was subsequently found to be V600K by 454.†Twelve samples subsequently reported as wild type, three as V600E2, and one as V600R by 454.