Data set on "In vitro growth kinetics of mesenchymal stem cells in cytotoxicity tests using ultra-diluted Viscum album

This dataset consists of three Excel.xlsx format spreadsheets. These are Morphology
and Quantification of MSC, Viability assays (MTT Colorimetric Assay and quantification of MSC).xlsx,
Apoptosis Cell Assay.xlsx.

Dataset
Morphology and Quantification of MSC.xlsx shows data on the impact of different VA concentrations on the D1D2
dynamization and the impact of its Diluent on MSC viability after 72 hours of in
vitro culture, through cell quantification analysis. The number of cells
was maintained after in vitro culturing for 72 hours regardless of the
diluent concentration used when compared to the control group. Similar MSC
morphological characteristics over 24, 48, and 72 hours of in vitro
culture were observed between the control group and the group at 10µL/mL of VA.
Data from the cell viability
test were subjected to normality analysis using the Shapiro-Wilk test. They
were then evaluated for differences between the experimental groups by the
paired Wilcoxon test. Data were subjected to regression analysis (PROC REG) for
the relevant polynomial regressions.

Dataset of Viability
assays MTT Colorimetric Assay shows data on the MSC were cultured in 96-well plates containing 5x10-4
cells/mL in 100µl of base MEDIUM at 37.5 °C, 5% CO2, for 24 hours
for cell stabilization and adhesion. This procedure was performed in double, so
half plates would be used for MTT assay and the other half for quantification.
After this period, the in vitro culture base MEDIUM was replaced, and
the evaluation factor, which contained the diluent and/or VA D1D2 at different concentrations, was added to
each group. MSC from all experimental groups and one
untreated group (Control) were cultured for a further 48 hours. The results of the MTT assays were analyzed
using Graph Prism 7.04 software by the Tukey test for multiple comparisons.

Dataset
Apoptotic cell assay labeling was
performed using the Annexin-V) consists of MSC samples from the groups:
Control, Diluent 10µL/mL, and VA 10µL/mL and 20μL/mL after in vitro
culture. A total of
92.0, 90.9, 96.2, and 91.5% of intact cells were obtained in the Control,
Diluent, VA (10µL/mL), and VA (20µL/mL) groups, respectively. Regarding the
percentages of MSC in initial apoptosis, 8.1, 0.5, 1.6, and 2.3% were obtained
in the Control, Diluent, VA (10µL/mL), and VA (20µL/mL) groups, respectively.
Additionally, the percentages of cells in final apoptosis were 0.1, 6.7, 1.7,
and 5.4% in the Control, Diluent, VA (10µL/mL), and VA (20µL/mL) groups,
respectively. The number of cells in necrosis was below 2% in all treatments
analyzed. The population of intact
cells in early apoptosis, late apoptosis, and necrosis was evaluated.