HoxB4 in hematopoietic stem cells. Mus musculus
收藏NIAID Data Ecosystem2026-03-07 收录
下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA136389
下载链接
链接失效反馈官方服务：
资源简介：
Ectopic expression of homeodomain transcription factor HoxB4 in mouse ES cells enhances in vitro development of hematopoietic stem cells (HSCs). Overall design: Mouse ES cells were differentiated for 6 days in EBs, then c-Kit+CD41+ hematopoietic precursor cells were isolated, plated onto OP9 cells, and transduced with the GFP control or 3×FLAG-tagged HoxB4 retrovirus. At day 12 of the co-culture with OP9 cells, c-Kit+ progenitor cells overexpressing HoxB4 were collected by magnetic-activated cell sorting (MACS) and subjected to a ChIP-chip and microarray analysis. The purity of the recovered c-Kit+ cells was more than 95% as judged by flow cytometry.
创建时间：
2011-05-23
