Whole genome sequencing of Arabidopsis mutant mdg5

Manganese is an essential trace element for plant growth and development, participating in crucial processes such as photosynthesis, protein synthesis, lipidsynthesis, enzyme activity, and defense against oxidative stress. As a vital componentof the photosystem II (PSII) oxygen-evolving complex in plants, manganese isindispensable for photosynthesis. However, other functions of manganese withinchloroplasts remain unclear. To uncover unknown manganese-associated activitieswithin chloroplasts, our laboratory performed EMS chemical mutagenesis on theArabidopsis manganese uptake-defective mutant nramp1 to establish a mutant library. We screened and identified a series of manganese-deficient sensitive mutants namedmdg (Mn-dependent defective growth). This study conducted phenotypic andphysiological analysis on two new yellow leaves mutantmdg5nramp1, as well as cloning and functional analysis of the target genes.