Quantification of total and unintegrated (UF) HIV DNA copy number in five different samples.

aThe results were obtained dividing the sum of the copy number from a total of eight replicates (two 0.5 µg replicates in the 1st qPCR and six 0.5 µg replicates in the 2nd qPCR) by 4 and expressed as HIV DNA copy number/µg of DNA.
bTaking into account the WBC number, the results can be reported as copies/ml of blood with the formula: (copies/µg DNA)×WBC no. per ml/142857 cells, assuming that 142857 cells are present in one µg of DNA. WBC counts/µl are 3980 (sample 1), 7480 (sample 2), 8660 (sample 3), 5610 (sample 4), and 6620 (sample 5).
cTaking into account %CD4+, the results can be reported as copies/104 CD4+ with the formula: [(copies/µg DNA)/(CD4+/WBC×142857 WBC)]×104. The %CD4+ are: 15.0% (sample 1), 3.2% (sample 2), 12.9% (sample 3), 6.8% (sample 4), and 6.0% (sample 5).
Quantification of total and unintegrated (UF) HIV DNA copy number in five different samples.