Neuronal calcium, RBC velocities and fUS responses to odorant stimuli in the mouse olfactory bulb

Dataset containing 2 HDF5 files, one per mouse. It is intended to be used to test our Transfer Function computing software: https://gitlab.com/AliK_A/buildtf and described in Aydin et al. (submitted).

Each file contains acquisitions of responses to odorant stimuli in the olfactory bulb made with :

two-photon linescan microscopy (for Ca2+ and RBC velocity);

functional ultrafast ultrasound, acquired from a coronal plane.

HDF5 files tree is as follows :

Data type

Raw : straight out of our extraction software, no specific treatment applied;

Aligned : every acquisition has been aligned so that the odor delivery matches the 10 s mark. Acquisitions have also been interpolated to be meaned;

Delta : aligned acquisitions are subtracted with the baseline value (between 5 and 10 s);

DetaOverBSL : aligned acquisitions are subtracted and then divided with the baseline value.

Data source

Ca : calcium data from GCamP6f expressed in the mitral cells dendritic tufts;

RBC : RBC velocities in a capillary near the calcium recording site, simultaneously acquired;

FUS : fUS data, coronal plane. Only in FUS folder is two different folders then : High and Lowspeed, corresponding to different filter for fUS treatment,  > 80Hz and 10-30Hz respectively.

Stimulation type : Odorant_Quantity_Duration

Odorant type, either Iso Amyl Acetate (AA) or Ethyl tiglate (ET);

Odor quantity : measured and calibrated in volt with a photo-ionizator;

Odor duration : from 5 s down to 120 ms, a single sniff for a mouse.

Ca2+ : Calcium

fUS : functional ultrafast ultrasound

RBC : Red Blood Cell