Neutralization of TNF-α does not reverse inhibition induced by hemozoin.
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Erythroid cultures were pre-incubated with 25µg/ml of the neutralising antibody to TNF-α, InfliximAb (Centocor, Horsham, USA) on day 0 of the erythropoietin dependent stage of culture. After 1 hour hemozoin (Hz) and TNF-α were added at 6µg/ml and 10ng/ml respectively. The number of live erythroid CD235a positive events acquired (out of a total of 10, 000) by flow cytometry on day 14 is shown. Data from a representative experiment of 3 is shown.
创建时间：
2013-02-21
