Data for: Cytosolic bacterial pathogens activate TLR pathways in tumors that synergistically enhance STING agonist cancer therapies

Bacterial pathogens that invade the eukaryotic cytosol are distinctive tools for fighting cancer, as they preferentially target tumors and can deliver cancer antigens to MHC-I. Cytosolic bacterial pathogens have undergone extensive preclinical development and human clinical trials, yet the molecular mechanisms by which they activate innate immunity in tumors are unclear. We report that intratumoral delivery of phylogenetically distinct cytosolic pathogens, including Listeria, Rickettsia, and Burkholderia species, elicited anti-tumor responses in established, poorly immunogenic melanoma and lymphoma in mice. We were surprised to observe that although the bacteria required entry to the cytosol, the anti-tumor responses were largely independent of the cytosolic sensors cGAS and STING and instead required TLR signaling. Combining pathogens with STING agonists elicited profound, synergistic anti-tumor effects with complete responses in >80% of mice after a single dose. Small-molecule TLR..., Tumors were injected on the day that they measured approximately 6 x 6 x 2.5 mm in each direction. Tumor volumes are reported as ellipsoids using the formula: V = (π/6)ABC. For any days not measured, tumor volumes were averaged between days of measurement. If animals were euthanized, tumor volumes were measured until the last mouse from each group was euthanized., , # Data for: Cytosolic bacterial pathogens activate TLR pathways in tumors that synergistically enhance STING agonist cancer therapies

[https://doi.org/10.5061/dryad.n2z34tn5z](https://doi.org/10.5061/dryad.n2z34tn5z)

## Description of the data and file structure

Figures in this manuscript fall into 3 categories: 1) tumor volume, 2) mouse survival, and 3) type I interferon (IFN-I) production.

**1) Tumor volume: Figures 1A, 1B, 1C, 1D, 1E, 2A, 2B, 3A-3D, 3F-3J, 4A, 4B, 4D, 4E, 5A-F, 6B, 7A**

The way to interpret these data is that the Y axis is tumor volume, and the X axis is time. Each column of data is an individual mouse. Mice were injected with the anti-cancer agents on day 0, when tumor volumes measured approximately 6 x 6 x 2.5 mm in each direction. Tumor volumes are reported as ellipsoids using the formula: *V* = (π/6)*ABC*. For any days not measured, tumor volumes were averaged between days of measurement. If animals were euthanized, tumor volumes were measured until the last...,