Kinetic analysis of DAT-mCherry FRAPa.

aPerformed on transfected SH-SY5Y cells as described (Materials and methods; Fig. 2) with Kinetic Analysis module of Zeiss AIM software. bK of fluorescence recovery (presented as mean ± SEM) calculated for a single exponential from background corrected fluorescence intensity at each time (I(t)) according to: [I(t) = IPOST–((I∞–IPOST)*exp(−t*K))] where IPOST = fluorescence intensity immediately following bleaching period and I∞ = plateau of fitted recovery curve (I∞ normalized to 1 for calculations). cMobile fraction of DAT-mCherry (FM) determined according to FM = I∞ – IPOST. dImmobile fraction of DAT-mCherry (FI) determined according to FI = IPRE – I∞, where IPRE = fluorescence intensity immediately prior to initiation of bleaching. Data are compiled from three experiments conducted independently on five cells per condition (n = 15) and are presented as mean ± SEM and analyzed by one-way ANOVA followed by Dunnet's post-hoc analysis for difference from DAT alone control (*** = p