Gene expression data from THP-1 cells exposed to exosomes from acetaminophen-treated or vehicle-treated hepatocytes or corresponding mock- or medium-controls

In the present study we examined the effect of hepatocyte-derived exosomes (HDE) on innate immune cells by treating a human monocyte cell line (THP-1 cells) with exosomes collected from the culture medium of acetaminophen (APAP)-treated and untreated primary human hepatocytes and comparing the cytokine response to THP-1 cells treated with mock- and medium-controls. We profiled the gene expression changes observed in the HDE-exposed monocytes prior to LPS stimulation. Findings from our research suggest that HDE may be essential for maintaining immune homeostasis. Twenty-four THP-1 samples were analyzed representing N=1 per donor-specific treatment from N=5 hepatocyte donors except 1 media-only sample from 1 donor that was > 3 SD away from the mean on a PCA plot suggesting that these samples may also be aberrant and were therefore omitted from analysis. Because the mock-control sample from 1 donor was omitted from the array, the mock-APAP data for this donor was duplicated for the purposes of HDE vs mock analysis.