Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Tuft_after_irr]

The goal of this study was to understand the role of thymic tuft cells in thymic regeneration following irradiation-induced acute involution. For this purpose we treated WT mice with sublethal irradiation, sorted tuft cells from their thymi 3 days after treatment when the thymus begins its recovery, and performed bulk RNA Seq. Young female WT mice were treated with sublethal irradiation 3 days before sacrifice, and then thymic tuft cells were isolated and mRNA profiles were generated by deep sequencing using the MARSseq method. 4-5 replicates were used. The control was untreated age-matched WT mice.