Engineering Drawings and CAD Models for "A Stopped-Flow Instrument for Millisecond Timescale Reaction Monitoring on a Standard NMR Spectrometer"

Engineering drawings and CAD models for: A Stopped-Flow Instrument for Millisecond Timescale Reaction Monitoring on a Standard NMR Spectrometer ABSTRACT: We report a new stopped-flow instrument designed for initiation and analysis of rapid reactions using standard NMR spectrometers and probes. The instrument is capable of kinetic measurements on mixing-initiated reactions with half-lives as short as 5 milliseconds. Design features of the instrument are discussed, with high-speed video footage, pressure and NMR line-shape data used to characterise mixing and stopping of reagent flows. The reactants are thermally and magnetically equilibrated prior to initiation of mixing and transport of the nascent reaction to the active volume for application of the NMR pulse sequence, ensuring near-quantitative results. NMR data acquisition and processing require special considerations for fast reactions, and simulations were carried out to investigate the effect of composite instrument dead-times on the observed rate constant. The kinetics of base-mediated hydrolysis of methyl formate (1H) and pentafluorophenyl boronic acid (19F) are employed to demonstrate the capability of the stopped-flow instrument for the reproducible generation of NMR data from fast reactions. Full details of the design and construction of the instrument, together with engineering and electronics drawings, are provided as supporting information. The system requires no permanent modifications to the spectrometer or console.