Data from: Phormia regina allele report

Genetic structure of blow fly (Diptera: Calliphoridae) populations has
remained elusive regardless of high relatedness within wild-caught
samples. The aims of this research were to determine if the implementation
of a high-resolution spatiotemporal sampling design would reveal latent
genetic structure among blow fly populations and to elucidate
environmental impacts on structure. Adult female black blow flies, Phormia
regina (Meigen), were collected from nine urban parks in Indiana, USA over
three years. Fly gut DNA was genotyped at six microsatellite loci, with
subsequent amplification and sequencing of vertebrate mitochondrial DNA
from the same source. Flies were also screened for vertebrate fecal
metabolites. Latent clustering revealed four genetic groups which were
interpreted as 11 distinct temporal populations. An analysis of molecular
variance of temporal populations revealed stronger genetic differentiation
(FST = 0.048, F’ST = 0.664) relative to geographic populations (FST =
0.009, F’ST = 0.241). Mean kinship within temporal populations was higher
than expected in a panmictic population (R = 0.032 ± 0.088). Weather
conditions (i.e., wind speed, precipitation, humidity, temperature) and
vertebrate resource availability in the local environment significantly
impacted the observed genetics of P. regina. Twenty-five vertebrate
species were detected from flies, and 16% of flies collected in 2016 –
2017 tested positive for vertebrate feces, suggesting many varied
resources are important for maintaining high gene flow among geographic
populations. A complex interplay between biotic and abiotic factors, as
well as the flies’ own extensive dispersal abilities, seems to drive the
strong temporal structure of this species in the Midwestern US.