Differential gene expression between immature and mature enterocyte like-Caco2 cells

Human Caco2 intestinal cells represent an in vitro model of human enterocytes. These cells are able to acquire a phenotype of mature polarized enterocytes when they are maintained in transwell culture system for 2-3 weeks.The goal of this study was to analyze the differences in mRNA expression profiles between Caco2 cells at a early stage of their differentiation (day 8) and at a more advanced stage of their differenciation (day 16) into absorptive enterocytes. A 3' Digital Gene expression (DGE) RNA sequencing was performed to determine mRNA expression profiles. 3' DGE RNA sequencing was performed on 4 samples of immature (day 8) and mature (day 16) enterocyte like-Caco2 cells.