IO3 panel assay characterization

Performance figures of merit in fit-for-purpose validation experiments for the multiplexed assay panel using frozen tissue and plasma matrices. The "curveFOM" tab contains response curve results describing the curve fit (correlation coefficient, R squared, slope), upper and lower limits of quantification, and linear dynamic range. The "repeatabilityFOM" tab contains repeatability of peptide measurement in each matrix reported as the intra-assay (within day) and inter-assay (between day) CV at three concentrations of spiked peptide (low, medium, high). The intra-assay and inter-assay CVs are also reported for peptides measured above the LLOQ at endogenous levels in the tissue and plasma matrices. The "stabilityFOM" tab contains the stability of the peptides in processed samples. Stability is presented by the percent difference in samples processed and stored in the autosampler for 24 hours or analyzed after 2 freeze-thaw cycles compared to immediate analysis.