Metabolomic profiles of acute and chronic ambient hydrogen sulfide exposure in a mouse model

Hydrogen sulfide (H2S) is an environmental toxicant of health concern following acute or chronic human exposures. Male 6-8 week-old C57BL/6J mice were exposed by whole-body inhalation to 1000 ppm H2S for 45 min and euthanized at 5 min and 72 h for acute exposure. For subchronic study, mice were exposed to 5 ppm H2S 2 h/day, 5 days/week for 5 weeks. The brainstem was removed for metabolomic analysis. The metabolomics analyses consisted of three assays, (1) primary metabolism by GC-TOF MS, (2) biogenic amines (hydrophilic compounds) by HILIC-MS/MS and (3) lipidomics by RPLC-MS/MS. Metabolomics were performed in West Coast Metabolomics Center, University of California at Davis, CA, USA. 348, 311, and 565 known metabolites were detected and analyzed by primary metabolism, biogenic amines, and lipidomic metabolomics assays. 33, 19, and 46 metabolites were increased at 5 min and 72 h post acute H2S exposures and subchronic ambient H2S exposures, respectively, compared to room air control grou..., Exposure paradigmHydrogen sulfide (H2S) is an environmental toxicant of health concern following acute or chronic human exposures. Male 6-8 week-old C57BL/6J mice were exposed by whole-body inhalation to 1000 ppm H2S for 45 min and euthanized at 5 min and 72 h for acute exposure. For subchronic study, mice were exposed to 5 ppm H2S 2 h/day, 5 days/week for 5 weeks. The brainstem was removed for metabolomic analysis. 
Metabolomics analysisThe metabolomics analyses consisted of three assays, (1) primary metabolism by GC-TOF MS, (2) biogenic amines (hydrophilic compounds) by HILIC-MS/MS and (3) lipidomics by RPLC-MS/MS. Metabolomics were performed in West Coast Metabolomics Center (WCMC), University of California at Davis, CA, USA. Raw data were normalized by the standard normalization in WCMC. , , # Metabolomic profiles of acute and chronic ambient hydrogen sulfide exposure in a mouse model

\# General information

\## This README file was generated on 20204-01-04 by Dongsuk Kim.

\## Author Information

A. Principal Investigator Contact Information

Name: Wilson Rumbeiha

Institution: University of California at Davis

Email:

B. Associate or Co-investigator Contact Information

Name: Dongsuk Kim

Institution: University of California at Davis

Email:

\# Dataset contents:

This data set contains 3 normalized metabolomics data in brainstem following acute and subchronic hydrogen sulfide exposures.

\# SHARING/ACCESS INFORMATION

1\. Licenses/restrictions placed on the data: CC0 1.0 Universal (CC0 1.0) Public Domain

2\. Links to publications that cite or use the data:

Dong-Suk Kim, Cristina MS Maldonado, Cecilia Giulivi, and Wilson K Rumbeiha (2024). Metabolomic signatures of brainstem in mice following acute and chronic hydrogen sulfide exposure.

3\. Links to other publicly...